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ICMR performs genome characterisation of monkeypox cases, identifies 3 sub-clusters among A.2 lineage in India

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New Delhi: The Indian Council of Medical Research (ICMR) has analyzed the complete genome sequences of confirmed Kerala and Delhi monkeypox cases between July and August 2022.

He has found three subgroups among the A.2 lineage: the first group Kerala (n5) and Delhi (n2) aligned with USA-2022 ON674051.1; while the second from Delhi (n3) was aligned with USA-2022 ON675438.1 and the third group consists of the UK, USA and Thailand, in the study conducted by ICMR-NIV Pune.

According to the study, all MPXV sequences recovered from India covering 90 to 99 percent of the genome belong to clade IIb lineage A.2.

“All the MPXV sequences recovered from India covering 90 to 99 percent of the genome belong to the A.2 lineage of clade IIb. The A.2 MPXV lineage divided into three subgroups; first group Kerala n5, Delhi n2 aligned with USA-2022 ON674051.1; while the second from Delhi n3 aligned with USA-2022 ON675438.1 and the third consists of the UK, USA and Thailand. The recent update in the MPXV lineage designated the five Kerala sequences as A.2.1,” the ICMR study revealed, emphasizing the need for improved genomic surveillance to understand the mutation and its link.

During the period from July to August 2022, clinical samples, i.e. oropharyngeal swab (OPS), nasopharyngeal swab (NPS), lesion scab and lesion fluids were submitted from 96 suspected cases of monkeypox from 18 states and 3 Union Territories to ICMR-National Institute of Virology, Pune, India for diagnosis of Monkeypox.

Clinical samples from all cases were analyzed by real-time PCR specific for monkeypox. Of these, five cases from Kerala and Delhi were found to be positive for MPXV. All monkeypox-negative cases were also tested for varicella zoster virus (VZV) and enterovirus (EV)-specific real-time PCR.

Out of 114 cases, MPXV infection was confirmed in ten cases from India using real-time PCR specific for Orthopox and Monkeypox. In addition, the detection of negative monkeypox cases indicated the presence of VZV and EV by real-time PCR. The ten confirmed cases of monkeypox were three men and two women from New Delhi with no history of international travel; while five men were from Kerala with a travel history from the United Arab Emirates (UAE) to India.

All the cases were immunocompetent without comorbidities with a mean age of 31 years and presented with a short prodromal phase of fever, myalgia, and vesicular-pustular lesions, mainly in the genital area, face, trunk, and extremities.

“Of all, nine cases had firm nontender lymphadenopathy at one or more sites (inguinal, cervical, submental, submandibular, retroauricular), while one case showed no lymphadenopathy. All cases have recovered without complications except for one case from Kerala who succumbed to infection after acute onset encephalitis,” the ICMR said.

“MPXV sequences from India were grouped into two subgroups; 7 sequences (Kerala n5, Delhi n2) aligned with strain USA-2022 ON674051.1 and UK-2022 OP331335.1 formed the first group. In this subset, five sequences from Kerala were designated A.2.1 based on lineage-defining mutations at position C 25072 T, A 140492 C, C 179537 T. Two sequences from Delhi lack all three mutations, thus which are still defined in the A.2 lineage,” said the ICMR study.

The study further added: “While mutations defining the A.2.1 lineage were observed to be missing in all 3 sequences of the second Delhi subgroup; aligned with strain USA-2022 ON675438.1. The third subgroup consists of the MPXV sequences obtained from UK-2022 OP331336.1, USA-2021 ON676707.1, and three sequences from Thailand-2022”.

(IANS)


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Apple threatens to withhold Twitter from App Store: Musk

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San Francisco: Twitter CEO Elon Musk said Apple has “threatened to withhold” the micro-blogging platform from its App Store without giving a reason.

Musk’s accusation came shortly after he tweeted late Monday that Apple has “mostly stopped advertising” on Twitter following a survey that asked users whether the iPhone maker should “publish all shares of censorship that it has taken that affect its customers”.

In a series of tweets, Musk said: “Apple also threatened to remove Twitter from its App Store, but won’t tell us why.

“Did you know that Apple puts a 30 percent secret tax on everything you buy through its App Store?”

Several users expressed their thoughts on Musk’s claim.

While one user commented, “Apple doesn’t support free speech,” another said, “because it’s violating the rules about moderating extremist content.”

Meanwhile, Yoel Roth, who resigned from Twitter as head of trust and safety earlier this month, said that while Musk perpetuates the lack of legitimacy through his impulsive changes and lengthy tweet pronouncements about the platform’s rules, Twitter it now faces close scrutiny from Apple and Google. app stores

In a New York Times article amid the #RIPTwitter trend on social media, Roth said that “Twitter will have to balance its new owner’s goals with the practical realities of Apple’s and Google’s internet life, a no-brainer.” easy for employees who have chosen to stay.

“And when I left the company, the calls from the application review teams had already started,” Roth wrote.

(IANOS)


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After furore for slurring women, Ramdev regrets and apologises

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Mumbai: Just 72 hours after his comments about women sparked a furor, a chastened yoga guru, Swami Ramdev, expressed his regret and apologized for his comments.

Ramdev emailed Maharashtra State Commission for Women Chairman Rupali Chakankar in response to her letter on Friday demanding an explanation of his remarks within 72 hours.

Confirming the developments, the MSCW boss reported that Ramdev sent him the reply, expressing regret and offering an apology, but also stating that his comments were allegedly twisted out of context.

“We have received your response to our notice… However, if there are any further objections or complaints, we will conduct a full investigation and obtain the full video recording of the show made last week,” Chakankar warned, while speaking to IANS. .

Addressing a free women’s yoga training program in Thane, Ramdev Baba blurted out to the mostly women gathering: “Women look good in sarees, they look great in salwar suits and, in My opinion, they look good even if they are not wearing anything.

At the time, Ramdev was flanked by Balasahebanchi MP Shrikant Shinde Shiv Sena Thane, son of Prime Minister Eknath Shinde, and singer Amruta Fadnavis, wife of Bharatiya Janata Party Deputy Chief Minister Devendra Fadnavis and other prominent personalities.

The blatant statements prompted calls for Ramdev to “slap, kick and admonish” them from various locations in Maharashtra and other parts of India.

Agitated top leaders from various parties, including Sanjay Raut, Dr. Manisha Kayande, Kishore Tiwari, Mahesh Tapase, women activists like Aparna Malikar, Trupti Desai and others, pounced on Ramdev, seeking an unconditional apology, framing him for demoting women and other demands. even as the MSCW slapped a notice.

(IANOS)


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Uorfi calls Chetan Bhagat a ‘pervert’ after he questions her dress sense

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Mumbai: Internet personality Uorfi Javed clashed with popular writer Chetan Bhagat over the latter’s comment calling her a “distraction” to children. Although Bhagat later clarified that he had been quoted out of context, Worfi went after him, all guns blazing.

Uorfi took to her Instagram Stories and shared a series of her chats that had gone viral a few years ago during the #MeToo campaign. He was later accused of sexual misconduct by author and yoga instructor Ira Trivedi.

After bringing the allegations against Bhagat to the attention of his followers, Uorfi reacted to the author’s comment: “Guys, let’s not forget how many women accused him during the #MeToo case!!”

He added that men like Bhagat only blame women and said there was no need to drag her into the conversation and make comments about the type of clothing she chooses to wear.

“Men like him will always blame women rather than accept their own shortcomings!! Just because you’re a pervert doesn’t mean it’s the girl’s fault or what she’s wearing! Uorfi said.

“Unnecessarily dragging myself into a conversation, commenting on how my clothes distract kids is such a fucked up thing to say! Isn’t texting young girls distracting to them though? She continued.

Uorfi also pointed out how Bhagat’s comment showed that his way of thinking was quite old-fashioned.

“Stop promoting rape culture you psychos out there! Blaming women’s clothing for men’s behavior is so 80s. Mr. Chetan Bhagat. Who was distracting you when you texted girls half your age? Uorfi said. “Always blame the opposite sex and never accept your own shortcomings or failings! People like you are cheating young people, not me. Encourage men to blame women or their clothes when they are to blame.

The former ‘Bigg Boss OTT’ contestant said all this in a video she shared, where she asked what was the need for the ‘Two States’ author to bear her name at a literature festival, despite the fact that she is not a writer, nor of his age.

“I don’t understand the need to mention my name at a literature festival. I’m not an author. Also, you said that the young man is distracted by looking at my photos. Put that aside. First, tell me why you, who are twice the age of youth, would send messages to girls half your age. Wasn’t that a distraction? Were your marriage and children not affected by it? Worfi asked.

Bhagat also took to his Twitter account to clarify that he was being questioned unnecessarily for what he had not said.

“I told the guys to focus on fitness and their running and not waste time on Instagram,” Bhagat said. “Apparently, that’s not right! So they cut out my statement, said it out of context, the headline added things I never said, and made an age discrimination click-bait piece,” he added.

(IANOS)


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